Fluorescein Sodium
Fluorescein sodium
CAS: 518-47-8
Molecular Formula: C20H13NaO5
Fluorescein Sodium - Names and Identifiers
Fluorescein Sodium - Physico-chemical Properties
| Molecular Formula | C20H13NaO5 |
| Molar Mass | 356.31 |
| Density | 0.579[at 20℃] |
| Melting Point | 320 °C |
| Water Solubility | 500 g/L (20 ºC) |
| Solubility | H2O: soluble1mg/mL |
| Vapor Presure | 2.133hPa |
| Appearance | Powder |
| Color | Rust-orange |
| Odor | Odorless |
| Maximum wavelength(λmax) | ['491nm, 493.5nm, 490nm'] |
| Merck | 14,4159 |
| BRN | 3833041 |
| pKa | 2.2, 4.4, 6.7(at 25℃) |
| PH | 8.3 (10g/l, H2O, 20℃) |
| Storage Condition | Store at +5°C to +30°C. |
| Stability | Stable. |
| MDL | MFCD00167039 |
Fluorescein Sodium - Risk and Safety
| Hazard Symbols | Xi - Irritant |
| Risk Codes | R36 - Irritating to the eyes R36/37/38 - Irritating to eyes, respiratory system and skin. |
| Safety Description | S22 - Do not breathe dust. S24/25 - Avoid contact with skin and eyes. S37/39 - Wear suitable gloves and eye/face protection S26 - In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. S36/37/39 - Wear suitable protective clothing, gloves and eye/face protection. S27 - Take off immediately all contaminated clothing. |
| WGK Germany | 1 |
| RTECS | LM5425000 |
| FLUKA BRAND F CODES | 3 |
| TSCA | Yes |
| HS Code | 32041200 |
| Toxicity | LD50 in mice, rats (mg/kg): 4738, 6721 orally (Yankel, Loux) |
Fluorescein Sodium - Reference
| Reference Show more | 1. Xiao Yunfeng, Sheng Wei, Liu Jian. Safety Evaluation of 0.01% atropine sulfate eye drops in experimental animals [J]. Northern Pharmacy 2020 17(07):143-144+196. 2. Cao Yong Zhao Moming Zhao Tiantian Cui Chun Zhao Qiang Zhong Feng Yunzi. Analysis of functional components and activity evaluation of different black tea extracts [J]. Food Science, 2017, 38(18):54-59. 3. Gao Xiong, Lin Xiaorong, et al. Isolation, purification and antioxidant activity of three kinds of polyphenols in maitake leaf tea [J]. Science and Technology for the food industry 2020 v.41;No.445(05):37-45. 4. Zhou Duan, Liu Shumin, Huang Huihua. Comparison of antioxidant capacity of tea extracts with different fermentation degrees and study on antioxidant activity of tea polyphenols cells [J]. Food Science and Technology 2014 039(009):216-222. 5. Wang Shaomei. Analysis of antioxidant components in Pu-erh tea based on the extraction of foreign substances [J]. Journal of Shandong Agricultural University (Natural Science Edition),2019,50(02):319-322. 6. Huang Pingqing, Gao Lili, Yu Yingchao, etc. Preparation and quality evaluation of L-carnitine thermosensitive in situ gel [J]. Chinese Journal of Pharmacy, 2019(6). 7. Liu Yang, Ma Huiqiang, Liu Dayong, etc. Comparison of the effect of invasive resin and pit and fissure sealant in the treatment of early caries [J]. Journal of Tianjin Medical University, 2018. 8. Chen Ting-Qiang, Liu Shumin, Huang Huihua. Study on the difference of functional components and antioxidant capacity between green tea and black tea extracts [J]. Modern Food Science and Technology, 2014(10):141-146. 9. Dan Li, Cao Yong, Wang Hua, et al. Correlation between antioxidant activity and functional components of tea extracts in vitro [J]. Food and machinery, 2018. 10. Wang Qian, He Ping, Lin Ruge, etc. Structure, composition analysis and functional activity of egg white protein from duck egg [J]. Science and Technology of food industry, 2020, v.41;No.448(08):73-79. 11. Chen Xi-Miao, Li Mei-ying, Xu Qiu-Li, et al. Changes of polyphenols and antioxidant activity of Hawthorn during simulated gastrointestinal digestion in vitro [J]. Food Science, 2019, 040(005): 31-37. 12. Yan, Wei, et al. "In vitro degradation of pure magnesium-the synergistic influences of glucose and albumin." Bioactive materials 5.2 (2020): 318-333.https:// doi.org/10.1016/j. Bioactma. 2020.02.015 13. [IF = 6.475] Ying Wu et al."Comparison and screening of bioactive phenolic compounds in different blueberry cultivars: Evaluation of anti-oxidation and α-glucosidase inhibition effect."Food Res Int. 2017 Oct;100:312 14. [IF=14.593] Wei Yan et al."In vitro degradation of pure magnesium―the synergetic influences of glucose and albumin."Bioact Mater. 2020 Jun;5:318 15. [IF=7.182] Chengxiao Wang et al."Dissolvable microneedles based on Panax notoginseng polysaccharide for transdermal drug delivery and skin dendritic cell activation."Carbohyd Polym. 2021 May;:118211 16. [IF=5.875] Luyao Zheng et al."Novel skin permeation enhancers based on amino acid ester ionic liquid: Design and permeation mechanism."Int J Pharmaceut. 2020 Feb;576:119031 17. [IF=4.545] Huimin Wu et al."Study on the potential effective ingredients of Xiaosheng prescription for dry eye disease."Biomed Pharmacother. 2020 Jul;127:110051 18. [IF=3.765] Feng Suqin et al."In-vitro and in situ assessment of the efflux of five antidepressants by breast cancer resistance protein."J Pharm Pharmacol. 2019 Jun;71(7):1133-1141 19. [IF=4.24] Bo zu et al."Structural identification and antioxidant potential evaluation of pomelo vinegar polyphenols." Food Biosci. 2022 Jun;47:101674 Note: For some products, our company can only provide some information, we do not guarantee the authority of the information provided, only for the reference and exchange of customers. storage conditions: 2-8 ℃ sensitivity: easy moisture absorption solubility: H2O: 1 mg/ml. Soluble in water and ethanol Appearance: orange-red powder Boiling Point: 620.8°C at 760 mmHg Melting Point: 320°C fluorescence: λex 460 nm; λem 515 nm fluorescein is a xanthine dye that is commonly used as a labeling reagent in biology. Fluorescein sodium salt is used as a solute to study microvascular permeability. It has been used as a test molecule to study blood barrier penetration. |
Fluorescein Sodium - Standard
Authoritative Data Verified Data
This product is 9-(o-c-phenyl)-6-hydroxy-3h-goxon-3-one disodium salt. The content of C20H10Na2O5 should be between 98.0% and 102.0% based on the water content.
Last Update：2024-01-02 23:10:35
Fluorescein Sodium - Trait
Authoritative Data Verified Data
- This product is orange-red powder or a block with slight metallic luster, which is orange-red powder after grinding.
- This product is soluble in water, slightly soluble in ethanol.
Last Update：2022-01-01 13:38:44
Fluorescein Sodium - Differential diagnosis
Authoritative Data Verified Data
- take 1 drop of the aqueous solution (1-2000) of this product, and point it on the filter paper to generate yellow spots, and place it in the bromine vapor while it is wet, and then contact with ammonia vapor after 1 minute, the spots turned dark pink.
- the aqueous solution of this product shows strong fluorescence, which is still very obvious after being diluted with a large amount of water; However, after acid is added to make it acidic, the fluorescence disappears; Alkali is added to make it alkaline, fluorescence reappeared.
- The infrared absorption spectrum of this product should be consistent with that of the control (Spectrum set 273). In case of inconsistency, it is advisable to add 0.lg of the product, add 0.lml of water, stir with a glass rod to completely dissolve, and dry at 105°C for 4 hours before determination.
- identification reaction of sodium salt after burning and ashing of this product (General rule 0301).
Last Update：2022-01-01 13:38:45
Fluorescein Sodium - Exam
Authoritative Data Verified Data
alkalinity
take 0.20g of this product, Add 10ml of water to dissolve it, and determine it according to law (General rule 0631). The pH value should be 7.0~9.0.
Related substances
take the appropriate amount of this product, precision weighing, plus solvent [acetonitrile-water (15:85 )] Dissolved and quantitatively diluted to make a solution containing 0.5mg per 1ml, as a test solution; Take 1ml of precision measurement, put it in a 200ml measuring flask, dilute it to the scale with the above solvent, shake it, and use it as a control solution; Take resorcinol (impurity I). The control substance and the phthalic acid (impurity II) control substance were precisely weighed, dissolved with the above solvent and quantitatively diluted to prepare a mixed solution containing 2.5UG each in 1ml as a control solution. According to the high performance liquid chromatography (General rule 0512) test, using octanosilane bonded silica gel as filler (×, 5um), the column temperature was 30 ° C, with phosphate solution (take potassium dihydrogen phosphate 1. 22G, dissolved in water and diluted to 1000ml, adjust the pH value to 20 with phosphoric acid) as mobile phase A, acetonitrile as mobile Phase B, the detection wavelength was 220nm, and the gradient elution was carried out according to the following table. The sample solution, the control solution and the reference solution were injected with 20 u1 respectively into the liquid chromatograph, and the chromatograms were recorded. In the chromatogram of the reference solution, the separation degree between the impurity I peak and the impurity II peak should be greater than 5.0. If there are chromatographic peaks in the chromatogram of the test solution that are consistent with the retention time of impurity I or impurity II in the chromatogram of the reference solution, the peak area shall not be greater than the peak area of impurity I or impurity II in the chromatogram of the reference solution (0.5%); The peak area of other individual impurities shall not be greater than 0.6 times (0.3%) of the main peak area of the control solution, the sum of the peak areas of other impurities shall not be greater than the main peak area of the control solution (0.5%). The peaks in the chromatogram of the test solution which were 0.1 times smaller than the main peak area of the control solution were ignored.
nitride
take 0.10g of this product, add 50ml of water to dissolve, add 1 ml of dilute nitric acid, shake well, stand for 10 minutes, make the fluorescein sodium precipitate completely, filter until clear. Divide two portions of filtrate, 10ml for each portion, separate them into 50ml Nessler's colorimetric tubes, add one portion of water to make 40ml, add 1.0 of silver nitrate solution, shake well, and place in the dark for 10 minutes, if it is turbid, after filtering until clear, the filtrate was added with 7.0ml of human standard gasified sodium solution, water was added to 50ml, and the mixture was shaken to obtain a control solution. Another portion was made up to 40ml by adding water, 1.0ml of silver nitrate test solution was added, water was added to 50ml, and the mixture was shaken to obtain a test solution. Take the control solution and the test solution, place it in the dark for 5 minutes, and check it according to law (General 0801). The test solution should not be more concentrated (0.35%) than the control solution.
sulfate
take 0.20g of this product, add 100ml of water to dissolve, add 7ml of dilute hydrochloric acid, make the fluorescein sodium precipitate completely, filter until clear. Divide two portions of the filtrate, 25ml each, separate them into 50ml Nessler's colorimetric tubes, add water to make 40ml, add 5ml of 25% barium chloride solution, shake, and place in the dark for 10 minutes, if it is turbid, filtered until clear, the filtrate was added to 2.5ml of standard potassium sulfate solution, water was added to 50ml, and shaken to serve as a control solution. Another portion was added with water to make 40ml, 25% barium chloride solution was added to 5ml, water was added to 50ml, and the mixture was shaken to obtain a test solution. Take the control solution and the test solution, place in the dark for 10 minutes, and check according to law (General rule 0802). The test solution should not be more concentrated (0.50%) than the control solution.
moisture
take this product, according to the determination of moisture (General 0832 first method 1), the water content shall not exceed 17.0%.
zinc salt
take 0.10g of this product, Add 10ml of saturated aqueous solution of sodium chloride to dissolve, add 2ml of dilute hydrochloric acid, shake, filter, add 1ml of potassium ferrocyanide test solution to the filtrate, and no turbidity.
Last Update：2022-01-01 13:38:46
Fluorescein Sodium - Content determination
Authoritative Data Verified Data
measured by high performance liquid chromatography (General 0512).
chromatographic conditions and system suitability test
silica gel bonded with eighteen alkyl silane was used as the filler; Acetonitrile-0.1% phosphoric acid solution (30:70) was used as the mobile phase; The detection wavelength was 232nm. The degree of separation between the sodium fluorescein peak and the adjacent impurity peaks shall be as required.
assay
take about 25mg of this product, precision weighing, put it in 50ml measuring flask, add water to dissolve and dilute to the scale, shake, take 2ml, put it in 100ml measuring flask, dilute to scale with water, shake well, inject 20u1 into human liquid chromatograph with precision, record chromatogram; Take about 25mg of fluorescein sodium reference substance, determine with the same method. According to the external standard method to calculate the peak area, that is.
Last Update：2022-01-01 13:38:47
Fluorescein Sodium - Category
Authoritative Data Verified Data
diagnostic medication.
Last Update：2022-01-01 13:38:47
Fluorescein Sodium - Storage
Authoritative Data Verified Data
sealed storage.
Last Update：2022-01-01 13:38:47
Fluorescein Sodium - Fluorescein sodium injection
Authoritative Data Verified Data
This product is a sterile aqueous solution of sodium fluorescein. The fluorescein sodium (C20H10Na2O5) content shall be between 90.0% and 110.0% of the labeled amount.
trait
This product is orange-red clear liquid.
identification
- take this product, according to fluorescein sodium under the identification of (1), (2) test, showed the same reaction.
- in the chromatogram recorded under the content determination item, the retention time of the main peak of the test solution should be consistent with the retention time of the main peak of the reference solution.
examination
- the pH value should be 8.0 to 9.8 (General 0631).
- appropriate amount of related substances of this product is quantitatively diluted with solvent [acetonitrile-water (15: 85)] to prepare a solution containing 0.5mg of sodium fluorescein per 1 ml as a test solution; test according to the method under the item sodium fluorescein related substances. If there are chromatographic peaks in the chromatogram of the test solution that are consistent with the retention time of impurity I or impurity II in the chromatogram of the reference solution, the peak area shall not be greater than the peak area of impurity I or impurity II in the chromatogram of the reference solution (0.5% ) ; The peak area of other individual impurities shall not be greater than 0.6 times (0.3%) of the main peak area of the control solution, the sum of the peak areas of other impurities shall not be greater than the main peak area of the control solution (0.5%). The peaks in the chromatogram of the test solution which were 0.1 times smaller than the main peak area of the control solution were ignored.
- pyrogen to take this product, according to the law (General rule 1142), the dose of rabbit body weight per lkg injection of 250mg, should comply with the provisions.
- others should comply with the relevant provisions under injection (General 0102).
Content determination
take an appropriate amount of this product (about 200mg equivalent to sodium fluorescein), put it in a 200ml measuring flask, dilute it with water to the scale, shake it, take 2ml for precision measurement, put it in a 200ml measuring flask, diluted with water to the scale, shake, as a test solution, according to the method under the determination of sodium fluorescein content, obtained.
category
Same as fluorescein sodium.
specification
(l)3ml:0.3g (2)3ml:0.6g
storage
sealed storage.
Last Update：2022-01-01 13:38:48
Fluorescein Sodium - Reference Information
| color index | 45350 |
| pH indicator color change ph range | Pink uorescence (4.0) to green uorescence (6.0) |
| main applications | Nanolithography, nanoparticles, thin films, fuel cells, inks, laser, colored bubbles, cleansing products, identifying leaks in coolant, suntanning agent, hair dyes, cosmetics, dermatology, dental material, diagnosis of diabetic retinopathy, antitumor agent |
| LogP | 0.34 |
| EPA chemical information | Information provided by: ofmpub.epa.gov (external link) |
| overview | fundus Fluorescein angiography (FFA) is one of the important methods for diagnosis and examination of fundus diseases, which is of great significance for diagnosis and differential diagnosis of fundus diseases, guiding laser treatment, prognosis judgment and curative effect observation. Sodium fluorescein is a diagnostic drug for fundus angiography. It cannot stain the normal corneal epithelium, but it can dye the damaged corneal epithelium green, which can show corneal damage, ulcers and other diseases. Sodium fluorescein injection is a contrast agent used for FFA. It is a carbohydrate dye. After it enters the blood circulation, it 80% combines with serum protein and red blood cells, and is 20% free in the blood, does not participate in the body's metabolism, and does not interact with the tissue. It is firmly combined and has little toxicity. It is mainly excreted in the urine through the kidneys, and the rest is excreted through the biliary tract. It is a safer contrast agent. However, in the process of clinical imaging examination, there are still individual subjects who may have local or systemic adverse reactions, and a few serious cases may even be life-threatening. |
| properties | sodium fluorescein is odorless and hygroscopic. Soluble in water, the solution is yellow-red with strong yellow-green fluorescence, disappears after acidification, and reappears after neutralization or alkalization, slightly soluble in ethanol, almost insoluble in chloroform and ether. Water solution and plasma isotonic. |
| Preparation | Sodium fluorescein is generally obtained from the heat condensation of resorcinol and phthalic anhydride to form a salt. It contains a lot of impurities, mainly unreacted resorcinol and phenolphthalein and yadine yellow. |
| Application | Use sodium fluorescein as a fluorescent tracer to study the blood-brain barrier (BBB) and blood-spinal barrier (BSCB) in rodent models Permeability. Using this dye as a probe substrate, the organic anion transport polypeptide (OATP)-mediated drug transport in hepatocytes was studied. Sodium fluorescein was also applied to the recovery of solute in situ into bone by fluorescence after photofading. The sodium fluorescein salt is used as a solute to study microvascular permeability. It has been used as a test molecule to study blood barrier penetration. |
| use | is the same as fluorescein. |
| toxic substance data | information provided by: pubchem.ncbi.nlm.nih.gov (external link) |
Last Update：2024-04-10 22:29:15
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